The Resource Angiogenesis : in vitro systems, edited by David Cheresh, (electronic resource)

Angiogenesis : in vitro systems, edited by David Cheresh, (electronic resource)

Label
Angiogenesis : in vitro systems
Title
Angiogenesis
Title remainder
in vitro systems
Statement of responsibility
edited by David Cheresh
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently.* Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the ""builder's manual"" for essential techniques--a one-stop shop that eliminates needless searc
Member of
Is Subseries of
Cataloging source
MiAaPQ
Dewey number
612.13
Illustrations
  • illustrations
  • plates
Index
index present
Language note
English
LC call number
QP106.6
LC item number
.A64 2008
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Cheresh, David A
Series statement
Methods in enzymology,
Series volume
v. 443
http://library.link/vocab/subjectName
  • Neovascularization
  • Blood-vessels
Label
Angiogenesis : in vitro systems, edited by David Cheresh, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Cover; Copyright Page; TOCContents; Contributors; Preface; Volumes in Series; CHChapter 1: Approaches for Studying Angiogenesis-Related Signal Transduction; 1. Introduction; 2. Angiogenesis; 3. Phosphorylation of eNOS on Ser1179; 4. Measuring Released NO by No-Specific Chemiluminescence; 5. Concluding Remarks; References; CHChapter 2: Physiologic Stress-Mediated Signaling in the Endothelium; 1. Introduction; 2. Parallel Plate Flow System; 3. Cone and Plate Flow System; 4. Cyclic Stretching of the Endothelium; Acknowledgments; References
  • CHChapter 3: Endothelial Cell Adhesion and Migration1. Introduction; 2. Cell Preparation; 3. Preparation of Well-Defined Surfaces; 4. Preparation of Polyacrylamide Substrates for Cell Adhesion Studies; 5. Quantifying Cell Adhesion; 6. Quantifying Endothelial Cell Migration; References; CHChapter 4: An Optimized Three-Dimensional In Vitro Model for the Analysis of Angiogenesis; 1. Overview; 2. Fibrin Bead Assay; 3. Immunocytochemistry of Angiogenic Sprouts In Vitro; 4. Retroviral Transduction of EC; 5. Laser Capture Microdissection; 6. Discussion; References
  • CHChapter 5: In Vitro Three Dimensional Collagen Matrix Models of Endothelial Lumen Formation During Vasculogenesis and Angiogenesis1. Introduction; 2. In Vitro Models of Vasculogenesis and Angiogenesis in 3D Collagen Matrices; 3. Molecular/Genetic Manipulation of ECs; 4. Rho GTPase Activation Assay During EC Tube Morphogenesis in 3D Collagen Matrices; 5. Biochemical Analysis of Interactions Between Cdc42 and its Downstream Effectors During EC Lumen and Tube Formation in 3D Colla; 6. Conclusions; Acknowledgments; References
  • CHChapter 6: In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels1. Introduction; 2. Maintenance of ES Cells; 3. Generation of Transgenic ES Cell Lines; 2. Concluding Remarks; Acknowledgments; References; CHChapter 7: The Aortic Ring Model of Angiogenesis; 1. Introduction; 2. Aortic Ring Angiogenesis Model: Basic Protocol; 3. Protocols for Analysis of Aortic Cultures; 4. Modifications of Basic Protocol; 5. Troubleshooting; 6. Concluding Remarks; References; CHChapter 8: An Assay System for In Vitro Detection of Permeability in Human ""Endothelium""
  • 1. Introduction2. Generation of a Human Endothelium Model for Permeability Studies; 3. Conclusion; Acknowledgments; References; CHChapter 9: Assays of Transendothelial Migration In Vitro; 1. Introduction; 2. Methods for Investigating Leukocyte Transendothelial Migration Under Static or Flow Conditions In Vitro; Acknowledgments; References; CHChapter 10: Cell Mechanics at Multiple Scales; 1. Introduction; 2. Large-Scale Cell Stretching; 3. Magnetic Micromanipulation; 4. Fluorescence Laser Tracking Microrheology; 5. General Summary and Conclusion; Acknowledgments; References
  • CHChapter 11: Vascular Integrin Signaling
Dimensions
unknown
Extent
1 online resource (403 p.)
Form of item
online
Isbn
9780080921679
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)365561
  • (OCoLC)808726499
  • (SSID)ssj0000288802
  • (PQKBManifestationID)12069047
  • (PQKBTitleCode)TC0000288802
  • (PQKBWorkID)10384251
  • (PQKB)10616834
  • (MiAaPQ)EBC365561
  • (EXLCZ)991000000000553768
Label
Angiogenesis : in vitro systems, edited by David Cheresh, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Cover; Copyright Page; TOCContents; Contributors; Preface; Volumes in Series; CHChapter 1: Approaches for Studying Angiogenesis-Related Signal Transduction; 1. Introduction; 2. Angiogenesis; 3. Phosphorylation of eNOS on Ser1179; 4. Measuring Released NO by No-Specific Chemiluminescence; 5. Concluding Remarks; References; CHChapter 2: Physiologic Stress-Mediated Signaling in the Endothelium; 1. Introduction; 2. Parallel Plate Flow System; 3. Cone and Plate Flow System; 4. Cyclic Stretching of the Endothelium; Acknowledgments; References
  • CHChapter 3: Endothelial Cell Adhesion and Migration1. Introduction; 2. Cell Preparation; 3. Preparation of Well-Defined Surfaces; 4. Preparation of Polyacrylamide Substrates for Cell Adhesion Studies; 5. Quantifying Cell Adhesion; 6. Quantifying Endothelial Cell Migration; References; CHChapter 4: An Optimized Three-Dimensional In Vitro Model for the Analysis of Angiogenesis; 1. Overview; 2. Fibrin Bead Assay; 3. Immunocytochemistry of Angiogenic Sprouts In Vitro; 4. Retroviral Transduction of EC; 5. Laser Capture Microdissection; 6. Discussion; References
  • CHChapter 5: In Vitro Three Dimensional Collagen Matrix Models of Endothelial Lumen Formation During Vasculogenesis and Angiogenesis1. Introduction; 2. In Vitro Models of Vasculogenesis and Angiogenesis in 3D Collagen Matrices; 3. Molecular/Genetic Manipulation of ECs; 4. Rho GTPase Activation Assay During EC Tube Morphogenesis in 3D Collagen Matrices; 5. Biochemical Analysis of Interactions Between Cdc42 and its Downstream Effectors During EC Lumen and Tube Formation in 3D Colla; 6. Conclusions; Acknowledgments; References
  • CHChapter 6: In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels1. Introduction; 2. Maintenance of ES Cells; 3. Generation of Transgenic ES Cell Lines; 2. Concluding Remarks; Acknowledgments; References; CHChapter 7: The Aortic Ring Model of Angiogenesis; 1. Introduction; 2. Aortic Ring Angiogenesis Model: Basic Protocol; 3. Protocols for Analysis of Aortic Cultures; 4. Modifications of Basic Protocol; 5. Troubleshooting; 6. Concluding Remarks; References; CHChapter 8: An Assay System for In Vitro Detection of Permeability in Human ""Endothelium""
  • 1. Introduction2. Generation of a Human Endothelium Model for Permeability Studies; 3. Conclusion; Acknowledgments; References; CHChapter 9: Assays of Transendothelial Migration In Vitro; 1. Introduction; 2. Methods for Investigating Leukocyte Transendothelial Migration Under Static or Flow Conditions In Vitro; Acknowledgments; References; CHChapter 10: Cell Mechanics at Multiple Scales; 1. Introduction; 2. Large-Scale Cell Stretching; 3. Magnetic Micromanipulation; 4. Fluorescence Laser Tracking Microrheology; 5. General Summary and Conclusion; Acknowledgments; References
  • CHChapter 11: Vascular Integrin Signaling
Dimensions
unknown
Extent
1 online resource (403 p.)
Form of item
online
Isbn
9780080921679
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)365561
  • (OCoLC)808726499
  • (SSID)ssj0000288802
  • (PQKBManifestationID)12069047
  • (PQKBTitleCode)TC0000288802
  • (PQKBWorkID)10384251
  • (PQKB)10616834
  • (MiAaPQ)EBC365561
  • (EXLCZ)991000000000553768

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