The Resource DNA repair, edited by Judith Campbell, Paul Modrich, (electronic resource)

DNA repair, edited by Judith Campbell, Paul Modrich, (electronic resource)

Label
DNA repair
Title
DNA repair
Statement of responsibility
edited by Judith Campbell, Paul Modrich
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
This volume emphasizes the intracellular consequences of DNA damage, describing procedures for analysis of checkpoint responses, DNA repair in vivo, replication fork encounter of DNA damage, as well as biological methods for analysis of mutation production and chromosome rearrangements. It also describes molecular methods for analysis of a number of genome maintenance activities including DNA ligases, helicases, and single-strand binding proteins.*Part B of a 2-part series*Addresses DNA maintenance enzymes*Discusses damage signaling*Presents In vivo analysis of DNA
Member of
Is Subseries of
Cataloging source
MiAaPQ
Dewey number
572.86459
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP601
LC item number
.D63 2006
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Campbell, Judith L
  • Modrich, Paul
Series statement
Methods in enzymology
Series volume
v. 409, pt. B
http://library.link/vocab/subjectName
  • DNA repair
  • Enzymology
Label
DNA repair, edited by Judith Campbell, Paul Modrich, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographic references and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Cover Page; Editors-in-Chief; Title Page; Copyright Page; Table of Contents; Contributors to Volume 409; Volume in Series; Chapter 1: Overproduction and Purification of RFC-Related Clamp Loaders and PCNA-Related Clamps from Saccharomyces cerevisiae; Introduction; Overproduction of Complexes in Yeast; Extract Preparation and Purification; Discussion; Acknowledgments; References; Chapter 2: Functional Assays for Replication Protein A (RPA); Introduction; Purification of Recombinant RPA; DNA-Binding Assays; Gel-Binding Assays (Gel Mobility Shift and Helix-Destabilization)
  • Surface Plasmon ResonanceFluorescence Polarization; Other DNA-Binding Assays; RPA-Protein Interactions (Enzyme-Linked Immunosorbent Assay); Functional Assays for RPA; In Vivo Localization; In Vivo Localization by Indirect Immunofluorescence; Summary and Conclusion; References; Chapter 3: Human DNA Ligases I, III, and IV-Purification and New Specific Assays for These Enzymes; Introduction; Overexpression and Purification of Human DNA Ligases; DNA Ligation Assays; Biotinylated Linear DNA Substrates with Blocked DNA Ends; Linear DNA Substrates to Measure Intra- and Intermolecular DNA Joining
  • Ligation Assays Using Fluorescent DNA SubstratesConcluding Remarks; Acknowledgments; References; Chapter 4: Enzymatic Mechanism of the WRN Helicase/Nuclease; Expression and Purification of Recombinant WRN Protein; Characterization of WRN Helicase Activity; Helicase Assays; Preparation of Radiolabeled Duplex DNA Substrate; Preparation of Four-Stranded Oligonucleotide-Based Holliday Junction; Preparation of Three-Stranded Oligonucleotide-Based D-Loop; Radiometric Helicase Assay; Real-Time Fluorometric Helicase Assay; ATPase Assay; Gel Mobility Shift Assay to Measure DNA Binding
  • Exonuclease AssayEffects of Post-Translational Modifications on WRN Catalytic Activities; WRN Protein Interactions that Modulate WRN Catalytic Activity; Acknowledgments; References; Chapter 5: Analysis of the DNA Unwinding Activity of RecQ Family Helicases; Introduction; Preparation of Substrates; Analysis of Helicase Activity; Safety Issues; References; Chapter 6: Characterization of Checkpoint Responses to DNA Damage in Saccharomyces cerevisiae: Basic Protocols; General Introduction; Budding Analysis; Microcolony Assay; Visualization of the Mitotic Spindle; Nuclear Staining with DAPI
  • Flow CytometryAnalysis of Rad53 Phosphorylation; Acknowledgments; References; Chapter 7: Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage; Introduction; Chromatin Association of ATR, Rad17, Rad9, and RPA; Purification of ATR, ATRIP, and Reconstitution of the ATR-ATRIP Complex; Recruitment of ATRIP and ATR-ATRIP to RPA-Coated Single-Stranded DNA; Purification of Rad17 and 9-1-1 Complexes; Recruitment of 9-1-1 Complexes to Primed ssDNA by RPA and Rad17 Complexes; Conclusion; Acknowledgments; References
  • Chapter 8: Multiple Approaches to Study S. cerevisiae Rad9, a Prototypical Checkpoint Protein
Dimensions
unknown
Extent
1 online resource (613 p.)
Form of item
online
Isbn
9780080464671
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)274672
  • (OCoLC)476019875
  • (SSID)ssj0000603099
  • (PQKBManifestationID)11444306
  • (PQKBTitleCode)TC0000603099
  • (PQKBWorkID)10571713
  • (PQKB)10372744
  • (MiAaPQ)EBC274672
  • (EXLCZ)991000000000364450
Label
DNA repair, edited by Judith Campbell, Paul Modrich, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographic references and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Cover Page; Editors-in-Chief; Title Page; Copyright Page; Table of Contents; Contributors to Volume 409; Volume in Series; Chapter 1: Overproduction and Purification of RFC-Related Clamp Loaders and PCNA-Related Clamps from Saccharomyces cerevisiae; Introduction; Overproduction of Complexes in Yeast; Extract Preparation and Purification; Discussion; Acknowledgments; References; Chapter 2: Functional Assays for Replication Protein A (RPA); Introduction; Purification of Recombinant RPA; DNA-Binding Assays; Gel-Binding Assays (Gel Mobility Shift and Helix-Destabilization)
  • Surface Plasmon ResonanceFluorescence Polarization; Other DNA-Binding Assays; RPA-Protein Interactions (Enzyme-Linked Immunosorbent Assay); Functional Assays for RPA; In Vivo Localization; In Vivo Localization by Indirect Immunofluorescence; Summary and Conclusion; References; Chapter 3: Human DNA Ligases I, III, and IV-Purification and New Specific Assays for These Enzymes; Introduction; Overexpression and Purification of Human DNA Ligases; DNA Ligation Assays; Biotinylated Linear DNA Substrates with Blocked DNA Ends; Linear DNA Substrates to Measure Intra- and Intermolecular DNA Joining
  • Ligation Assays Using Fluorescent DNA SubstratesConcluding Remarks; Acknowledgments; References; Chapter 4: Enzymatic Mechanism of the WRN Helicase/Nuclease; Expression and Purification of Recombinant WRN Protein; Characterization of WRN Helicase Activity; Helicase Assays; Preparation of Radiolabeled Duplex DNA Substrate; Preparation of Four-Stranded Oligonucleotide-Based Holliday Junction; Preparation of Three-Stranded Oligonucleotide-Based D-Loop; Radiometric Helicase Assay; Real-Time Fluorometric Helicase Assay; ATPase Assay; Gel Mobility Shift Assay to Measure DNA Binding
  • Exonuclease AssayEffects of Post-Translational Modifications on WRN Catalytic Activities; WRN Protein Interactions that Modulate WRN Catalytic Activity; Acknowledgments; References; Chapter 5: Analysis of the DNA Unwinding Activity of RecQ Family Helicases; Introduction; Preparation of Substrates; Analysis of Helicase Activity; Safety Issues; References; Chapter 6: Characterization of Checkpoint Responses to DNA Damage in Saccharomyces cerevisiae: Basic Protocols; General Introduction; Budding Analysis; Microcolony Assay; Visualization of the Mitotic Spindle; Nuclear Staining with DAPI
  • Flow CytometryAnalysis of Rad53 Phosphorylation; Acknowledgments; References; Chapter 7: Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage; Introduction; Chromatin Association of ATR, Rad17, Rad9, and RPA; Purification of ATR, ATRIP, and Reconstitution of the ATR-ATRIP Complex; Recruitment of ATRIP and ATR-ATRIP to RPA-Coated Single-Stranded DNA; Purification of Rad17 and 9-1-1 Complexes; Recruitment of 9-1-1 Complexes to Primed ssDNA by RPA and Rad17 Complexes; Conclusion; Acknowledgments; References
  • Chapter 8: Multiple Approaches to Study S. cerevisiae Rad9, a Prototypical Checkpoint Protein
Dimensions
unknown
Extent
1 online resource (613 p.)
Form of item
online
Isbn
9780080464671
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)274672
  • (OCoLC)476019875
  • (SSID)ssj0000603099
  • (PQKBManifestationID)11444306
  • (PQKBTitleCode)TC0000603099
  • (PQKBWorkID)10571713
  • (PQKB)10372744
  • (MiAaPQ)EBC274672
  • (EXLCZ)991000000000364450

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