The Resource Methods in enzymology, Part B, Circadian rhythms and biological clocks, edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others]

Methods in enzymology, Part B, Circadian rhythms and biological clocks, edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others]

Label
Methods in enzymology, Part B, Circadian rhythms and biological clocks
Title
Methods in enzymology
Title number
Part B
Title part
Circadian rhythms and biological clocks
Statement of responsibility
edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others]
Contributor
Contributor
Editor
Subject
Genre
Language
  • eng
  • eng
Summary
Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock fun
Member of
Cataloging source
MiAaPQ
Dewey number
574.1925
Illustrations
  • illustrations
  • charts
  • plates
Index
index present
Language note
English
LC call number
TP248.E5
LC item number
.M484 2015
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Allada, Ravi
  • Sehgal, Amita
Series statement
Methods in Enzymology,
Series volume
Volume 552
http://library.link/vocab/subjectName
  • Enzymes
  • Circadian rhythms
  • Biological rhythms
Label
Methods in enzymology, Part B, Circadian rhythms and biological clocks, edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others]
Instantiates
Publication
Copyright
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references at the end of each chapters and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; Circadian Rhythms and Biological Clocks, Part B; Copyright; Contents; Contributors; Preface; Part I: Dissecting the Central Clock Circuit; Chapter One: Measuring Synchrony in the Mammalian Central Circadian Circuit; 1. Introduction; 1.1. What is synchrony?; 1.2. What is circadian synchrony?; 1.3. Goals of this review; 2. Monitoring SCN Rhythms with Cellular Resolution; 3. Isolating Data from Single Cells; 4. Defining a Rhythm; 4.1. Plotting rhythmic data for visual inspection; 5. Period Synchrony: Methods to Extract and Compare Periods Between Cells
  • 5.1. Do cells share the same period?6. Phase Synchrony: Methods to Extract and Compare Phase Relationships Between Cells; 7. Perturbations Reveal Synchronization Mechanisms; 8. Methods Awaiting Application in Circadian Biology; 9. Step-by-Step Instructions for Measuring Synchrony in SCN Slice; 9.1. Bioluminescence recordings using a charge-coupled device camera; 9.2. Image processing; 9.3. Single-cell tracking; 9.4. Data presentation; Acknowledgments; References; Chapter Two: Patch-Clamp Electrophysiology in Drosophila Circadian Pacemaker Neurons; 1. The Drosophila Circadian Network
  • 2. Circadian Control of Neuronal Activity2.1. Intrinsic currents; 2.2. Synaptic currents; 3. Methods for Patch-Clamp Electrophysiology; 3.1. Equipment; 3.2. Solutions; 3.3. Drosophila brain dissection; 3.4. Recordings; 3.5. Assessing the quality of the recording; 3.6. Relevant data in current clamp; 3.7. Relevant data in voltage clamp; 4. Conclusion; Acknowledgments; References; Chapter Three: Glial Cell Regulation of Rhythmic Behavior; 1. Introduction; 2. Studies of Glial Cell Function in Circadian Behavior and Sleep; 2.1. Glia and circadian behavior
  • 2.2. Mammalian astrocytes and the sleep homeostat2.3. Glia-neuron communication regulates Drosophila sleep; 2.4. Microglial clocks, synaptic strength, and sleep; 3. Potential Circadian Glia-Neuron Signaling Molecules; 3.1. Glial clocks and ATP rhythms; 3.2. Glial neurotrophic factors and cytokines in circadian behavior; 3.3. Secreted molecules mediating glia-neuron communication in Drosophila; 4. Molecular Genetic Strategies for Studying the Glial Regulation of Drosophila Rhythms; 4.1. Glia-selective genetic perturbation methods
  • 4.2. Glial expression profiling and cell-specific targeted genetic screens4.3. Glial microRNAs as regulators of rhythmicity; Appendix A. Protocol for TRAP Profiling of Fly Glial Cells; A.1. Bead preparation; A.2. Sample preparation; A.3. Immunoprecipitation; A.4. RNA extraction for RNA-seq library preparation; A.5. Reagents; References; Chapter Four: Neurophysiological Analysis of the Suprachiasmatic Nucleus: A Challenge at Multiple Levels; 1. Introduction; 2. Part I: Clock Mechanisms at the Cellular Level; 2.1. The molecular feedback loops and beyond; 2.2. Cytosolic oscillators
  • 2.3. Role of neuropeptides
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (432 p.)
Form of item
online
Isbn
9780128033814
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)1977755
  • (SSID)ssj0001550940
  • (PQKBManifestationID)16166997
  • (PQKBTitleCode)TC0001550940
  • (PQKBWorkID)14811452
  • (PQKB)11683764
  • (MiAaPQ)EBC1977755
  • (EXLCZ)993710000000357925
Label
Methods in enzymology, Part B, Circadian rhythms and biological clocks, edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others]
Publication
Copyright
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references at the end of each chapters and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; Circadian Rhythms and Biological Clocks, Part B; Copyright; Contents; Contributors; Preface; Part I: Dissecting the Central Clock Circuit; Chapter One: Measuring Synchrony in the Mammalian Central Circadian Circuit; 1. Introduction; 1.1. What is synchrony?; 1.2. What is circadian synchrony?; 1.3. Goals of this review; 2. Monitoring SCN Rhythms with Cellular Resolution; 3. Isolating Data from Single Cells; 4. Defining a Rhythm; 4.1. Plotting rhythmic data for visual inspection; 5. Period Synchrony: Methods to Extract and Compare Periods Between Cells
  • 5.1. Do cells share the same period?6. Phase Synchrony: Methods to Extract and Compare Phase Relationships Between Cells; 7. Perturbations Reveal Synchronization Mechanisms; 8. Methods Awaiting Application in Circadian Biology; 9. Step-by-Step Instructions for Measuring Synchrony in SCN Slice; 9.1. Bioluminescence recordings using a charge-coupled device camera; 9.2. Image processing; 9.3. Single-cell tracking; 9.4. Data presentation; Acknowledgments; References; Chapter Two: Patch-Clamp Electrophysiology in Drosophila Circadian Pacemaker Neurons; 1. The Drosophila Circadian Network
  • 2. Circadian Control of Neuronal Activity2.1. Intrinsic currents; 2.2. Synaptic currents; 3. Methods for Patch-Clamp Electrophysiology; 3.1. Equipment; 3.2. Solutions; 3.3. Drosophila brain dissection; 3.4. Recordings; 3.5. Assessing the quality of the recording; 3.6. Relevant data in current clamp; 3.7. Relevant data in voltage clamp; 4. Conclusion; Acknowledgments; References; Chapter Three: Glial Cell Regulation of Rhythmic Behavior; 1. Introduction; 2. Studies of Glial Cell Function in Circadian Behavior and Sleep; 2.1. Glia and circadian behavior
  • 2.2. Mammalian astrocytes and the sleep homeostat2.3. Glia-neuron communication regulates Drosophila sleep; 2.4. Microglial clocks, synaptic strength, and sleep; 3. Potential Circadian Glia-Neuron Signaling Molecules; 3.1. Glial clocks and ATP rhythms; 3.2. Glial neurotrophic factors and cytokines in circadian behavior; 3.3. Secreted molecules mediating glia-neuron communication in Drosophila; 4. Molecular Genetic Strategies for Studying the Glial Regulation of Drosophila Rhythms; 4.1. Glia-selective genetic perturbation methods
  • 4.2. Glial expression profiling and cell-specific targeted genetic screens4.3. Glial microRNAs as regulators of rhythmicity; Appendix A. Protocol for TRAP Profiling of Fly Glial Cells; A.1. Bead preparation; A.2. Sample preparation; A.3. Immunoprecipitation; A.4. RNA extraction for RNA-seq library preparation; A.5. Reagents; References; Chapter Four: Neurophysiological Analysis of the Suprachiasmatic Nucleus: A Challenge at Multiple Levels; 1. Introduction; 2. Part I: Clock Mechanisms at the Cellular Level; 2.1. The molecular feedback loops and beyond; 2.2. Cytosolic oscillators
  • 2.3. Role of neuropeptides
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (432 p.)
Form of item
online
Isbn
9780128033814
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)1977755
  • (SSID)ssj0001550940
  • (PQKBManifestationID)16166997
  • (PQKBTitleCode)TC0001550940
  • (PQKBWorkID)14811452
  • (PQKB)11683764
  • (MiAaPQ)EBC1977755
  • (EXLCZ)993710000000357925

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