The Resource Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering, by Kiyotaka Akabori, (electronic resource)

Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering, by Kiyotaka Akabori, (electronic resource)

Label
Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering
Title
Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering
Statement of responsibility
by Kiyotaka Akabori
Creator
Contributor
Author
Provider
Subject
Language
eng
Summary
This Thesis in biological physics has two components, describing the use of X-ray scattering techniques to study the structure of two different stacked lipid membrane systems. The first part focuses on the interaction between a short 11-mer peptide, Tat, which is part of the Tat protein in the HIV-1 virus. Although highly positively charged, the Tat protein has been shown to translocate through hydrocarbon lipid bilayers easily, without requiring the cell’s energy, which is counter to its Born self-energy. In this work Tat’s location in the headgroup region was demonstrated using a combined X-ray scattering and molecular dynamics approach. Bilayer thinning was observed as well as softening of different membrane mimics due to Tat. It was concluded that Tat’s headgroup location, which increases the area/lipid, and its bilayer softening likely reduce the energy barrier for passive translocation. The second part is a rigorous investigation of an enigmatic phase in the phase diagram of the lipid dimyristoylphosphatidylcholine (DMPC). The ripple phase has fascinated many researchers in condensed matter physics and physical chemistry as an example of periodically modulated phases, with many theoretical and simulation papers published. Despite systematic studies over the past three decades, molecular details of the structure were still lacking. By obtaining the highest resolution X-ray data so far, this work revealed the complex nature of the chain packing, as well as confirming that the major side is thicker than the minor side of the saw-tooth ripple structure. The new model shows that the chains in the major arm are tilted with respect to the bilayer normal and that the chains in the minor arm are slightly more disordered than all-trans gel-phase chains, i.e., the chains in the minor arm are more fluid-like. This work provides the highest resolution X-ray structure of the ripple phase to-date
Member of
http://library.link/vocab/creatorName
Akabori, Kiyotaka
Image bit depth
0
LC call number
  • QH601-602
  • QR77
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
SpringerLink
Series statement
Springer Theses, Recognizing Outstanding Ph.D. Research,
http://library.link/vocab/subjectName
  • Physics
  • Physical chemistry
  • Cell membranes
  • Crystallography
  • Membranes (Biology)
  • Physics
  • Membranes
  • Crystallography
  • Membrane Biology
  • Physical Chemistry
Label
Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering, by Kiyotaka Akabori, (electronic resource)
Instantiates
Publication
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction -- Lipid bilayers -- Tat peptide -- Pb¢ ripple phase -- Structural and Material Perturbations of Lipid Bilayers Due to HIV-1 Tat Peptide -- Introduction -- Materials and Methods -- Analysis of Molecular Dynamics Simulation Data -- Results -- Discussion -- Conclusions -- Ripple Phase -- Introduction -- Materials and Methods -- LAXS Data Reduction -- Results for Fhk Form Factors -- Models to Fit the Fhk and Obtain the Phase Factors -- Electron Density Profiles and Coarse Grained Bilayer Structure -- nGIWAXS: Results -- tWAXS: Results -- Thin Rod Model -- Combining WAXS and LAXS Results for the Major Arm -- Discussion -- Conclusion -- Appendices
Dimensions
unknown
Extent
XX, 168 p. 114 illus., 89 illus. in color.
File format
multiple file formats
Form of item
electronic
Isbn
9783319222103
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other control number
10.1007/978-3-319-22210-3
Other physical details
online resource.
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(DE-He213)978-3-319-22210-3
Label
Structure Determination of HIV-1 Tat/Fluid Phase Membranes and DMPC Ripple Phase Using X-Ray Scattering, by Kiyotaka Akabori, (electronic resource)
Publication
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction -- Lipid bilayers -- Tat peptide -- Pb¢ ripple phase -- Structural and Material Perturbations of Lipid Bilayers Due to HIV-1 Tat Peptide -- Introduction -- Materials and Methods -- Analysis of Molecular Dynamics Simulation Data -- Results -- Discussion -- Conclusions -- Ripple Phase -- Introduction -- Materials and Methods -- LAXS Data Reduction -- Results for Fhk Form Factors -- Models to Fit the Fhk and Obtain the Phase Factors -- Electron Density Profiles and Coarse Grained Bilayer Structure -- nGIWAXS: Results -- tWAXS: Results -- Thin Rod Model -- Combining WAXS and LAXS Results for the Major Arm -- Discussion -- Conclusion -- Appendices
Dimensions
unknown
Extent
XX, 168 p. 114 illus., 89 illus. in color.
File format
multiple file formats
Form of item
electronic
Isbn
9783319222103
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other control number
10.1007/978-3-319-22210-3
Other physical details
online resource.
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(DE-He213)978-3-319-22210-3

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