The Resource Two-component signaling systems, Part C, edited by Melvin I. Simon, Brian R. Crane, Alexandrine Crane, (electronic resource)

Two-component signaling systems, Part C, edited by Melvin I. Simon, Brian R. Crane, Alexandrine Crane, (electronic resource)

Label
Two-component signaling systems, Part C
Title
Two-component signaling systems
Title number
Part C
Statement of responsibility
edited by Melvin I. Simon, Brian R. Crane, Alexandrine Crane
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the ""two-component system."" This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock. * Includes time-tested core methods and new innovations applicable to any res
Member of
Is Subseries of
Cataloging source
MiAaPQ
Dewey number
  • 572.7
  • 572.8633
Illustrations
  • illustrations
  • plates
Index
index present
Language note
English
LC call number
QP517.C45
LC item number
T86 2010
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Simon, Melvin I
  • Crane, Brian R
  • Crane, Alexandrine
Series statement
  • Methods in enzymology,
  • Two-component signaling systems
Series volume
  • v. 471
  • pt. C
http://library.link/vocab/subjectName
  • Cellular signal transduction
  • Chemotaxis
Label
Two-component signaling systems, Part C, edited by Melvin I. Simon, Brian R. Crane, Alexandrine Crane, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; Methods in Enzymology Two-Component Signaling Systems, Part C; Copyright Page; Contents; Contributors; Chapter 1: Characterizing Cross-Talk In Vivo: Avoiding Pitfalls and Overinterpretation; Abstract; 1. Overview; 2. Sources of Cross-Talk; 3. Cross-Talk Suppression; 4. Transcriptional Reporters; 5. Response Regulator Localization; 6. Phosphatase Cross-Talk; 7. Signal Response in Cross-Talk Networks; 8. Concluding Remarks; Acknowledgments; References; Chapter 2: Inference of Direct Residue Contacts in Two-Component Signaling; Abstract; 1. Introduction; 2. Extraction Tools
  • 3. DCA: Direct Coupling AnalysisAppendix: Nonstandard Linear Algebra Functions; Acknowledgments; References; Chapter 3: Computational Modeling of Phosphotransfer Complexes in Two-Component Signaling; Abstract; 1. Introduction; 2. Methods; 3. Summary; Acknowledgments; References; Chapter 4: Kinetic Studies of the Yeast His-Asp Phosphorelay Signaling Pathway; Abstract; 1. Introduction; 2. Materials and Methods; 3. Conclusion; Acknowledgments; References; Chapter 5: Purification of MBP-EnvZ Fusion Proteins Using an Automated System; Abstract; 1. Introduction
  • 2. Comparison Between the Cytoplasmic Domains of E. coli and Typhi EnvZ Proteins3. Purification of the Recombinant MBP Proteins by FPLC; 4. SDS-PAGE Analysis of Recombinant Expressed MBP-EnvZc Protein; 5. Results; 6. Discussion; Acknowledgments; References; Chapter 6: Measurement of Response Regulator Autodephosphorylation Rates Spanning Six Orders of Magnitude; Abstract; 1. Overview of Response Regulator Autodephosphorylation; 2. Purification of Response Regulator Proteins; 3. General Considerations for Autodephosphorylation Assays; 4. Assay of Autodephosphorylation by Loss of 32P
  • 5. Assay of Autodephosphorylation by Fluorescence6. Assay of Autodephosphorylation by Pi Release; 7. Assay of Autodephosphorylation from Systems of Reactions; 8. Future Prospects; Acknowledgments; References; Chapter 7: Transmembrane Receptor Chimeras to Probe HAMP Domain Function; Abstract; 1. Introduction; 2. Design of Chimeras of HAMP Domains Fused to the AC Rv3645 Catalytic Domain; 3. Choice of Vector and Cloning Strategy; 4. Expression and Purification of the Chimeras; 5. AC Assay; 6. Example of Applications of the Method and Results; 7. Concluding Remarks; Acknowledgments; References
  • Chapter 8: Light-Activated Bacterial LOV-Domain Histidine KinasesAbstract; 1. Introduction; 2. Description of Method; 3. Concluding Remarks and Future Perspectives; Acknowledgments; References; Chapter 9: Characterization of Bacteriophytochromes from Photosynthetic Bacteria; Abstract; 1. Introduction; 2. Cloning BphP in Expression Vector; 3. Overexpression and Purification of BphPs; 4. Autophosphorylation; 5. Phosphotransfer; 6. Gel Mobility Shift Assay and DNase I Footprint; 7. Gene Disruption; 8. Analysis of the Photosynthetic Phenotypes of the BphP Mutants; 9. Photochemical Measurements
  • References
Dimensions
unknown
Edition
1st ed.
Extent
1 online resource (494 p.)
Form of item
online
Isbn
9781283020367
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)670208
  • (OCoLC)813310188
  • (SSID)ssj0000542153
  • (PQKBManifestationID)12253069
  • (PQKBTitleCode)TC0000542153
  • (PQKBWorkID)10510084
  • (PQKB)11623403
  • (MiAaPQ)EBC670208
  • (EXLCZ)992500000000003179
Label
Two-component signaling systems, Part C, edited by Melvin I. Simon, Brian R. Crane, Alexandrine Crane, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; Methods in Enzymology Two-Component Signaling Systems, Part C; Copyright Page; Contents; Contributors; Chapter 1: Characterizing Cross-Talk In Vivo: Avoiding Pitfalls and Overinterpretation; Abstract; 1. Overview; 2. Sources of Cross-Talk; 3. Cross-Talk Suppression; 4. Transcriptional Reporters; 5. Response Regulator Localization; 6. Phosphatase Cross-Talk; 7. Signal Response in Cross-Talk Networks; 8. Concluding Remarks; Acknowledgments; References; Chapter 2: Inference of Direct Residue Contacts in Two-Component Signaling; Abstract; 1. Introduction; 2. Extraction Tools
  • 3. DCA: Direct Coupling AnalysisAppendix: Nonstandard Linear Algebra Functions; Acknowledgments; References; Chapter 3: Computational Modeling of Phosphotransfer Complexes in Two-Component Signaling; Abstract; 1. Introduction; 2. Methods; 3. Summary; Acknowledgments; References; Chapter 4: Kinetic Studies of the Yeast His-Asp Phosphorelay Signaling Pathway; Abstract; 1. Introduction; 2. Materials and Methods; 3. Conclusion; Acknowledgments; References; Chapter 5: Purification of MBP-EnvZ Fusion Proteins Using an Automated System; Abstract; 1. Introduction
  • 2. Comparison Between the Cytoplasmic Domains of E. coli and Typhi EnvZ Proteins3. Purification of the Recombinant MBP Proteins by FPLC; 4. SDS-PAGE Analysis of Recombinant Expressed MBP-EnvZc Protein; 5. Results; 6. Discussion; Acknowledgments; References; Chapter 6: Measurement of Response Regulator Autodephosphorylation Rates Spanning Six Orders of Magnitude; Abstract; 1. Overview of Response Regulator Autodephosphorylation; 2. Purification of Response Regulator Proteins; 3. General Considerations for Autodephosphorylation Assays; 4. Assay of Autodephosphorylation by Loss of 32P
  • 5. Assay of Autodephosphorylation by Fluorescence6. Assay of Autodephosphorylation by Pi Release; 7. Assay of Autodephosphorylation from Systems of Reactions; 8. Future Prospects; Acknowledgments; References; Chapter 7: Transmembrane Receptor Chimeras to Probe HAMP Domain Function; Abstract; 1. Introduction; 2. Design of Chimeras of HAMP Domains Fused to the AC Rv3645 Catalytic Domain; 3. Choice of Vector and Cloning Strategy; 4. Expression and Purification of the Chimeras; 5. AC Assay; 6. Example of Applications of the Method and Results; 7. Concluding Remarks; Acknowledgments; References
  • Chapter 8: Light-Activated Bacterial LOV-Domain Histidine KinasesAbstract; 1. Introduction; 2. Description of Method; 3. Concluding Remarks and Future Perspectives; Acknowledgments; References; Chapter 9: Characterization of Bacteriophytochromes from Photosynthetic Bacteria; Abstract; 1. Introduction; 2. Cloning BphP in Expression Vector; 3. Overexpression and Purification of BphPs; 4. Autophosphorylation; 5. Phosphotransfer; 6. Gel Mobility Shift Assay and DNase I Footprint; 7. Gene Disruption; 8. Analysis of the Photosynthetic Phenotypes of the BphP Mutants; 9. Photochemical Measurements
  • References
Dimensions
unknown
Edition
1st ed.
Extent
1 online resource (494 p.)
Form of item
online
Isbn
9781283020367
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (EBL)670208
  • (OCoLC)813310188
  • (SSID)ssj0000542153
  • (PQKBManifestationID)12253069
  • (PQKBTitleCode)TC0000542153
  • (PQKBWorkID)10510084
  • (PQKB)11623403
  • (MiAaPQ)EBC670208
  • (EXLCZ)992500000000003179

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